PrimePCR™ Probe Assay: Crtap, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: Crtap, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Crtap, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0048746
Assay Design:   Exonic
Chromosome Location:   8:121734185-121734290question
Amplicon Length:   76
Splice Variants Targeted:   ENSRNOT00000075435 ENSRNOT00000065013 ENSRNOT00000025354 ENSRNOT00000041665 ENSRNOT00000011832 ENSRNOT00000041777 ENSRNOT00000040029 ENSRNOT00000037439 ENSRNOT00000048245 ENSRNOT00000041577 ENSRNOT00000005023 ENSRNOT00000012334 ENSRNOT00000031056 ENSRNOT00000022839 ENSRNOT00000026941 ENSRNOT00000014158 ENSRNOT00000006789 ENSRNOT00000042983 ENSRNOT00000022233 ENSRNOT00000017618 ENSRNOT00000075524 ENSRNOT00000005775 ENSRNOT00000038088 ENSRNOT00000054987 ENSRNOT00000059814 ENSRNOT00000035385 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Crtap
Gene Name:   Cartilage-associated protein precursor
Aliases:   Not Available
RefSeq:   NM_001108785
Ensembl:   ENSRNOG00000048343
Entrez:   363158
Chromosome Mapping:   8q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.290000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download