PrimePCR™ Probe Assay: HRAS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HRAS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HRAS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0033892
Assay Design:   Exonic
Chromosome Location:   11:533793-533922question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000397594 ENST00000397596 ENST00000311189 ENST00000451590 ENST00000417302

Gene Information

This gene belongs to the Ras oncogene family whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome a disease characterized by increased growth at the prenatal stage growth deficiency at the postnatal stage predisposition to tumor formation mental retardation skin and musculoskeletal abnormalities distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers including bladder cancer follicular thyroid cancer and oral squamous cell carcinoma. Multiple transcript variants which encode different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   HRAS
Gene Name:   v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Aliases:   C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1
RefSeq:   NC_000011.9 NT_009237.18 NG_007666.1
Ensembl:   ENSG00000174775
Entrez:   3265
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.650000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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