PrimePCR™ Probe Assay: HSD17B7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSD17B7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSD17B7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040759
Assay Design:   exonic
Chromosome Location:   1:162762534-162762640question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000339507 ENST00000418709 ENST00000437221 ENST00000301332 ENST00000301331 ENST00000409871 ENST00000343936 ENST00000361303 ENST00000435606 ENST00000367915 ENST00000254521 ENST00000367913 ENST00000367917 ENST00000284425 ENST00000253401 ENST00000374872 ENST00000374870 ENST00000367296 ENST00000295624 ENST00000367297 ENST00000367300 ENST00000272845 ENST00000439458 ENST00000264828 ENST00000347528 ENST00000289734 ENST00000265709 ENST00000379758 ENST00000396945 ENST00000396942 ENST00000352337 ENST

Gene Information

HSD17B7 encodes an enzyme that functions both as a 17-beta-hydroxysteroid dehydrogenase (EC 1.1.1.62) in the biosynthesis of sex steroids and as a 3-ketosteroid reductase (EC 1.1.1.270) in the biosynthesis of cholesterol (Marijanovic et al. 2003 [PubMed 12829805]).[supplied by OMIM May 2010]

Gene Symbol:   HSD17B7
Gene Name:   hydroxysteroid (17-beta) dehydrogenase 7
Aliases:   MGC12523, MGC75018, PRAP, SDR37C1
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000132196
Entrez:   51478
Chromosome Mapping:   1q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.770000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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