PrimePCR™ Probe Assay: RAPGEF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RAPGEF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAPGEF1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050446
Assay Design:   exonic
Chromosome Location:   9:134526226-134585009question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000372189 ENST00000372195 ENST00000438647 ENST00000427994 ENST00000372190

Gene Information

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis integrin-mediated signal transduction and cell transformation. Several alternatively spliced transcript variants of this gene have been described but the full-length nature of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   RAPGEF1
Gene Name:   Rap guanine nucleotide exchange factor (GEF) 1
Aliases:   C3G, DKFZp781P1719, GRF2
RefSeq:   NC_000009.11 NT_035014.4
Ensembl:   ENSG00000107263
Entrez:   2889
Chromosome Mapping:   9q34.3

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