PrimePCR™ Probe Assay: PSPC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSPC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSPC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051163
Assay Design:   exonic
Chromosome Location:   13:20346456-20346588question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000338910 ENST00000427943

Gene Information

This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA NEAT1/Men epsilon/beta and the Drosophila Behavior Human Splicing family of proteins which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene which is also located on chromosome 13 has been identified. [provided by RefSeq Aug 2011]

Gene Symbol:   PSPC1
Gene Name:   paraspeckle component 1
Aliases:   DKFZp566B1447, FLJ10955, PSP1
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000121390
Entrez:   55269
Chromosome Mapping:   13q12.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.920000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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