PrimePCR™ Probe Assay: PBX1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PBX1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PBX1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055020
Assay Design:   exonic
Chromosome Location:   1:164761888-164768950question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000340699 ENST00000420696 ENST00000559240 ENST00000367897 ENST00000559578 ENST00000560469 ENST00000496120 ENST00000468104 ENST00000560641 ENST00000482110 ENST00000558837 ENST00000559560 ENST00000540236 ENST00000401534 ENST00000540246

Gene Information

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis and required for skeletal patterning and programming. A chromosomal translocation t(1;19) involving this gene and TCF3/E2A gene is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   PBX1
Gene Name:   pre-B-cell leukemia homeobox 1
Aliases:   DKFZp686B09108, MGC126627
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000185630
Entrez:   5087
Chromosome Mapping:   1q23

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