PrimePCR™ Probe Assay: PPIP5K2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPIP5K2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPIP5K2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057856
Assay Design:   exonic
Chromosome Location:   5:102484875-102485003question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000586448 ENST00000255226 ENST00000381222 ENST00000381218 ENST00000381223 ENST00000321521 ENST00000507921 ENST00000358359 ENST00000414217 ENST00000576175 ENST00000586767 ENST00000570566 ENST00000587081

Gene Information

Inositol phosphates (IPs) and diphosphoinositol phosphates (PP-IPs) also known as inositol pyrophosphates act as cell signaling molecules. HISPPD1 has both IP6 kinase (EC 2.7.4.21) and PP-IP5 (also called IP7) kinase (EC 2.7.4.24) activities that produce the high-energy pyrophosphates PP-IP5 and PP2-IP4 (also called IP8) respectively (Fridy et al. 2007 [PubMed 17690096]).[supplied by OMIM Mar 2008]

Gene Symbol:   PPIP5K2
Gene Name:   diphosphoinositol pentakisphosphate kinase 2
Aliases:   FLJ21506, FLJ23463, HISPPD1, IP7K2, KIAA0433, VIP2
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000145725
Entrez:   23262
Chromosome Mapping:   5q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.310000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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