PrimePCR™ SYBR® Green Assay: OAS3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0028543


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PrimePCR™ PreAmp for SYBR® Green Assay: OAS3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: OAS3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0012667
Assay Design:		Intron-spanning
Chromosome Location:		12:113403772-113405285question
Amplicon Length:		96
Splice Variants Targeted:		ENST00000228928 ENST00000546973 ENST00000323881

Gene Information

This gene encodes an enzyme included in the 2' 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2' 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq Jul 2008]

Gene Symbol:		OAS3
Gene Name:		2'-5'-oligoadenylate synthetase 3, 100kDa
Aliases:		MGC133260, p100
RefSeq:		NC_000012.11 NT_009775.17
Ensembl:		ENSG00000111331
Entrez:		4940
Chromosome Mapping:		12q24.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.090000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.