PrimePCR™ Probe Assay: RANGAP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RANGAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RANGAP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027137
Assay Design:   Intron-spanning
Chromosome Location:   22:41645765-41647077question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000405486 ENST00000356244 ENST00000405383 ENST00000455915 ENST00000407260

Gene Information

RanGAP1 is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN but not of RAS by over 1000-fold. RanGAP1 is the immediate antagonist of RCC1 a regulator molecule that keeps RAN in the active GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins but is 88% identical to Fug1 the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq Jul 2008]

Gene Symbol:   RANGAP1
Gene Name:   Ran GTPase activating protein 1
Aliases:   Fug1, KIAA1835, MGC20266, SD
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100401
Entrez:   5905
Chromosome Mapping:   22q13

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