PrimePCR™ Probe Assay: INHBA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: INHBA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: INHBA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027657
Assay Design:   Intron-spanning
Chromosome Location:   7:41730111-41739699question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000242208 ENST00000442711

Gene Information

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore the beta A subunit forms a homodimer activin A and also joins with a beta B subunit to form a heterodimer activin AB both of which stimulate FSH secretion. Finally it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq Jul 2008]

Gene Symbol:   INHBA
Gene Name:   inhibin, beta A
Aliases:   EDF, FRP
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000122641
Entrez:   3624
Chromosome Mapping:   7p15-p13

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