PrimePCR™ Probe Assay: Birc5, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Birc5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Birc5, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0029624
Assay Design:   Intron-spanning
Chromosome Location:   11:117849786-117852749question
Amplicon Length:   135
Splice Variants Targeted:   ENSMUST00000081387 ENSMUST00000093906

Gene Information

This gene is a member of the inhibitor of apoptosis (IAP) gene family which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. In humans gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts have been identified in human that regulate this gene's expression. At least three transcript variants encoding distinct isoforms have been found for this gene although at least one of these transcript variants is a nonsense-mediated decay (NMD) candidate. [provided by RefSeq Jul 2008]

Gene Symbol:   Birc5
Gene Name:   Baculoviral IAP repeat-containing 5
Aliases:   AAC-11, Api4, TIAP, survivin40
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000017716
Entrez:   11799
Chromosome Mapping:   11|11 E2

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