PrimePCR™ Probe Assay: Nfkb1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Nfkb1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nfkb1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0025529
Assay Design:   intron_spanning
Chromosome Location:   2:259350292-259351746question
Amplicon Length:   91
Splice Variants Targeted:   ENSRNOT00000042316 ENSRNOT00000036838 ENSRNOT00000040511 ENSRNOT00000068674

Gene Information

gene encodes a 105 kD protein that can be processed to create a 50 kD protein; the larger protein is a Rel protein-specific transcription inhibitor and the smaller protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex [RGD, Feb 2006]

Gene Symbol:   Nfkb1
Gene Name:   Nuclear factor NF-kappa-B p105 subunit Nuclear factor NF-kappa-B p50 subunit
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000023258
Entrez:   81736
Chromosome Mapping:   2q43

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