PrimePCR™ Probe Assay: MYL6B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MYL6B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYL6B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040996
Assay Design:   exonic
Chromosome Location:   12:56547664-56547757question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000553066 ENST00000550443 ENST00000551834 ENST00000552568 ENST00000207437 ENST00000306726 ENST00000396137

Gene Information

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains two nonphosphorylatable alkali light chains and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in both slow-twitch skeletal muscle and in nonmuscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2010]

Gene Symbol:   MYL6B
Gene Name:   myosin, light chain 6B, alkali, smooth muscle and non-muscle
Aliases:   MLC1SA
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000196465
Entrez:   140465
Chromosome Mapping:   12q13.13

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