PrimePCR™ Probe Assay: HIST1H2BD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HIST1H2BD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HIST1H2BD, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041657
Assay Design:   exonic
Chromosome Location:   6:26158390-26158506question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000344147 ENST00000373714 ENST00000546109 ENST00000422613 ENST00000314332 ENST00000396984 ENST00000289316 ENST00000377777 ENST00000359985 ENST00000377733 ENST00000294119 ENST00000301935 ENST00000529640

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene which is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H2BD
Gene Name:   histone cluster 1, H2bd
Aliases:   H2B.1B, H2B/b, H2BFB, HIRIP2, MGC90432, dJ221C16.6
RefSeq:   NC_000006.11 NG_001335.1 NT_007592.15
Ensembl:   ENSG00000158373
Entrez:   3017
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.330000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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