PrimePCR™ Probe Assay: NME4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NME4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NME4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050228
Assay Design:   exonic
Chromosome Location:   16:449384-449628question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000397722 ENST00000454619 ENST00000382940 ENST00000219479 ENST00000433358 ENST00000450036

Gene Information

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates via a phosphohistidine intermediate between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family which includes NME4 (Milon et al. 1997 [PubMed 9099850]).[supplied by OMIM May 2008]

Gene Symbol:   NME4
Gene Name:   non-metastatic cells 4, protein expressed in
Aliases:   NDPK-D, NM23H4, nm23-H4
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103202
Entrez:   4833
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.800000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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