PrimePCR™ Probe Assay: RFC5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RFC5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RFC5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050637
Assay Design:   exonic
Chromosome Location:   12:118454597-118454691question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000435259 ENST00000504727 ENST00000394976 ENST00000338051 ENST00000414725 ENST00000461307 ENST00000207726 ENST00000314845 ENST00000533727 ENST00000534300 ENST00000458649 ENST00000528950 ENST00000426438 ENST00000298834 ENST00000349466 ENST00000359149 ENST00000263519 ENST00000599174 ENST00000537315 ENST00000484086 ENST00000420967 ENST00000454402 ENST00000392542 ENST00000535092 ENST00000229043 ENST00000388996 ENST00000519445 ENST00000201647 ENST00000540810 ENST00000375120 ENST00000304992 ENST

Gene Information

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC also named activator 1 is a protein complex consisting of five distinct subunits of 140 40 38 37 and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9. [provided by RefSeq May 2011]

Gene Symbol:   RFC5
Gene Name:   replication factor C (activator 1) 5, 36.5kDa
Aliases:   MGC1155, RFC36
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000111445
Entrez:   5985
Chromosome Mapping:   12q24.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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