PrimePCR™ Probe Assay: PLA2G4A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PLA2G4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLA2G4A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050960
Assay Design:   exonic
Chromosome Location:   1:186957522-186957625question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000367466 ENST00000442353

Gene Information

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids including prostaglandins and leukotrienes are lipid-based cellular hormones that regulate hemodynamics inflammatory responses and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq Jul 2008]

Gene Symbol:   PLA2G4A
Gene Name:   phospholipase A2, group IVA (cytosolic, calcium-dependent)
Aliases:   MGC126350, PLA2G4, cPLA2-alpha
RefSeq:   NC_000001.10 NG_012203.1 NT_004487.19
Ensembl:   ENSG00000116711
Entrez:   5321
Chromosome Mapping:   1q25

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