PrimePCR™ Probe Assay: RARA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RARA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RARA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051666
Assay Design:   exonic
Chromosome Location:   17:38506167-38508244question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000254066 ENST00000394089 ENST00000425707 ENST00000394086 ENST00000394081 ENST00000475125

Gene Information

This gene represents a nuclear retinoic acid receptor. The encoded protein retinoic acid receptor alpha regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development differentiation apoptosis granulopoeisis and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq Sep 2010]

Gene Symbol:   RARA
Gene Name:   retinoic acid receptor, alpha
Aliases:   NR1B1, RAR
RefSeq:   NC_000017.10 NG_027701.1 NT_010783.15
Ensembl:   ENSG00000131759
Entrez:   5914
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.860000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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