PrimePCR™ Probe Assay: CYBA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYBA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYBA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057838
Assay Design:   exonic
Chromosome Location:   16:88709817-88709928question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000458112 ENST00000262304 ENST00000423118 ENST00000567946 ENST00000434178 ENST00000487473 ENST00000398101 ENST00000484451 ENST00000265052 ENST00000453507 ENST00000398104 ENST00000204961 ENST00000269298 ENST00000555019 ENST00000315988 ENST00000555507 ENST00000372980 ENST00000373178 ENST00000240123 ENST00000252999 ENST00000168977 ENST00000593949 ENST00000064778 ENST00000338492 ENST00000368627 ENST00000368629 ENST00000298351 ENST00000361221 ENST00000375415 ENST00000375408 ENST00000452522 ENST

Gene Information

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD) that is characterized by the failure of activated phagocytes to generate superoxide which is important for the microbicidal activity of these cells. [provided by RefSeq Jul 2008]

Gene Symbol:   CYBA
Gene Name:   cytochrome b-245, alpha polypeptide
Aliases:   p22-PHOX
RefSeq:   NC_000016.9 NG_007291.1 NT_010542.15
Ensembl:   ENSG00000051523
Entrez:   1535
Chromosome Mapping:   16q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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