PrimePCR™ Probe Assay: SLIT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: SLIT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLIT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040976
Assay Design:   Intron-spanning
Chromosome Location:   10:98820458-98823262question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000453547 ENST00000399151 ENST00000393930 ENST00000310706 ENST00000393931 ENST00000266058 ENST00000371070 ENST00000314867 ENST00000371041 ENST00000448521 ENST00000440166 ENST00000452943 ENST00000468694 ENST00000494774 ENST00000432854 ENST00000490734 ENST00000562070 ENST00000341514 ENST00000343533 ENST00000350425 ENST00000341511 ENST00000265662 ENST00000371605 ENST00000561976 ENST00000389386 ENST00000424260 ENST00000263816 ENST00000448484 ENST00000249749 ENST00000343813 ENST00000264775 ENST

Gene Information

Description Not Available

Gene Symbol:   SLIT1
Gene Name:   slit homolog 1 (Drosophila)
Aliases:   MEGF4, MGC164811, SLIL1, SLIT-1, SLIT3
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000187122
Entrez:   6585
Chromosome Mapping:   10q23.3-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.140000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download