PrimePCR™ lncRNA® Probe Assay:TOP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Same primer pair as used in SYBR assay qhsaLID0104231

List Price:    $247.00
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PrimePCR™ IncRNA PreAmp for Probe Assay: TOP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for Probe Assay: TOP1, Human
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   lncRNA Expression
Unique Assay ID:   qhsaLIP0181471
Assay Design:   intron_spanning
Chromosome Location:   chr20:41061461-41076260question
Amplicon Length:   89
Refseq Transcripts Amplified:   NM_003286
Splice Variants Targeted:   ENST00000361337
LNCipedia Splice Variants Targeted:   lnc-PLCG1-3:1

Gene Information

This gene encodes a DNA topoisomerase an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq Jul 2008]

Gene Symbol:   TOP1
Gene Name:   topoisomerase (DNA) I
Aliases:   TOPI
RefSeq:   NC_000020.10 NG_012262.1 NT_011362.10
Ensembl:   ENSG00000198900
LNCipedia:   lnc-PLCG1-3
Entrez:   7150
Chromosome Mapping:   20q12-q13.1
Gene Strand:   Not Available

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