PrimePCR™ Probe Assay: Pde5a, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Pde5a, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Pde5a, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0029393
Assay Design:   intron_spanning
Chromosome Location:   2:246361197-246365710question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000019638

Gene Information

catalyzes the hydrolysis of cGMP to 5'-GMP; may regulate vascular relaxation; may be involved in establishment of cerebellar neural networks [RGD, Feb 2006]

Gene Symbol:   Pde5a
Gene Name:   CGMP-specific 3',5'-cyclic phosphodiesterase
Aliases:   Not Available
RefSeq:   NM_133584
Ensembl:   ENSRNOG00000014443
Entrez:   171115
Chromosome Mapping:   2q42

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