PrimePCR™ Probe Assay: CYP21A2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYP21A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP21A2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040178
Assay Design:   exonic
Chromosome Location:   6:32006913-32007147question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000436607 ENST00000448877 ENST00000379361 ENST00000383322 ENST00000383321 ENST00000448478 ENST00000434026 ENST00000456152 ENST00000452386 ENST00000418967 ENST00000478281 ENST00000471671 ENST00000435122 ENST00000448314 ENST00000452708 ENST00000547367 ENST00000547880 ENST00000547683 ENST00000322054

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP21A2
Gene Name:   cytochrome P450, family 21, subfamily A, polypeptide 2
Aliases:   CA21H, CAH1, CPS1, CYP21, CYP21B, MGC150536, MGC150537, P450c21B
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NG_007941.2 NG_008337.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167249.1
Ensembl:   ENSG00000231852
Entrez:   1589
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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