PrimePCR™ Probe Assay: GAPDH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GAPDH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GAPDH, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041396
Assay Design:   exonic
Chromosome Location:   12:6647267-6647413question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000229239 ENST00000396856 ENST00000396861 ENST00000396859 ENST00000396858

Gene Information

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2012]

Gene Symbol:   GAPDH
Gene Name:   glyceraldehyde-3-phosphate dehydrogenase
Aliases:   G3PD, GAPD, MGC88685
RefSeq:   NC_000012.11 NG_007073.2 NT_009759.16
Ensembl:   ENSG00000111640
Entrez:   2597
Chromosome Mapping:   12p13

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