PrimePCR™ Probe Assay: PRKCQ, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PRKCQ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRKCQ, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049490
Assay Design:   exonic
Chromosome Location:   10:6549448-6553083question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000263125 ENST00000397176 ENST00000539722

Gene Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1 and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq Jul 2008]

Gene Symbol:   PRKCQ
Gene Name:   protein kinase C, theta
Aliases:   MGC126514, MGC141919, PRKCT, nPKC-theta
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000065675
Entrez:   5588
Chromosome Mapping:   10p15

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