PrimePCR™ Probe Assay: RALGDS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RALGDS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RALGDS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053148
Assay Design:   exonic
Chromosome Location:   9:135973896-135974023question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000372050 ENST00000372047 ENST00000393160 ENST00000542690 ENST00000372062 ENST00000393157

Gene Information

Guanine nucleotide dissociation stimulators (GDSs or exchange factors) such as RALGDS are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM Nov 2010]

Gene Symbol:   RALGDS
Gene Name:   ral guanine nucleotide dissociation stimulator
Aliases:   FLJ20922, RGDS, RGF, RalGEF
RefSeq:   NC_000009.11 NT_035014.4
Ensembl:   ENSG00000160271
Entrez:   5900
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.000000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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