PrimePCR™ Probe Assay: FOS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FOS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FOS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053956
Assay Design:   exonic
Chromosome Location:   14:75746697-75746803question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000409032 ENST00000392770 ENST00000530277 ENST00000299333 ENST00000526094 ENST00000529383 ENST00000530828 ENST00000303562 ENST00000554617 ENST00000554212 ENST00000535987 ENST00000555242 ENST00000555686 ENST00000555672 ENST00000557139 ENST00000433366 ENST00000412198 ENST00000398706

Gene Information

The Fos gene family consists of 4 members: FOS FOSB FOSL1 and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family thereby forming the transcription factor complex AP-1. As such the FOS proteins have been implicated as regulators of cell proliferation differentiation and transformation. In some cases expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq Jul 2008]

Gene Symbol:   FOS
Gene Name:   FBJ murine osteosarcoma viral oncogene homolog
Aliases:   AP-1, C-FOS
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000170345
Entrez:   2353
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.540000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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