PrimePCR™ Probe Assay: ENPP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ENPP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ENPP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055475
Assay Design:   exonic
Chromosome Location:   6:132214235-132214383question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000360971

Gene Information

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification ossification of the posterior longitudinal ligament of the spine (OPLL) and insulin resistance. [provided by RefSeq Jul 2008]

Gene Symbol:   ENPP1
Gene Name:   ectonucleotide pyrophosphatase/phosphodiesterase 1
Aliases:   ARHR2, M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1
RefSeq:   NC_000006.11 NG_008206.1 NT_025741.15
Ensembl:   ENSG00000197594
Entrez:   5167
Chromosome Mapping:   6q22-q23

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