PrimePCR™ Probe Assay: EPAS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EPAS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EPAS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028462
Assay Design:   Intron-spanning
Chromosome Location:   2:46603772-46605069question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000263734

Gene Information

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq Nov 2009]

Gene Symbol:   EPAS1
Gene Name:   endothelial PAS domain protein 1
Aliases:   ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
RefSeq:   NC_000002.11 NG_016000.1 NT_022184.15
Ensembl:   ENSG00000116016
Entrez:   2034
Chromosome Mapping:   2p21-p16

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