PrimePCR™ Probe Assay: SIRT5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SIRT5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SIRT5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030171
Assay Design:   Intron-spanning
Chromosome Location:   6:13592055-13595767question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000379262 ENST00000379250 ENST00000359782 ENST00000397350

Gene Information

This gene encodes a member of the sirtuin family of proteins homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq Jul 2010]

Gene Symbol:   SIRT5
Gene Name:   sirtuin 5
Aliases:   FLJ36950, SIR2L5
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000124523
Entrez:   23408
Chromosome Mapping:   6p23

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