PrimePCR™ Probe Assay: KEAP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KEAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KEAP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031292
Assay Design:   Intron-spanning
Chromosome Location:   19:10597447-10599963question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000171111 ENST00000393623

Gene Information

This gene encodes a protein containing KELCH-1 like domains as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KEAP1
Gene Name:   kelch-like ECH-associated protein 1
Aliases:   INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000079999
Entrez:   9817
Chromosome Mapping:   19p13.2

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