PrimePCR™ Probe Assay: ENPP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ENPP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ENPP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031928
Assay Design:   Intron-spanning
Chromosome Location:   8:120575207-120577140question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000259486 ENST00000427067 ENST00000522167 ENST00000522826 ENST00000075322

Gene Information

The protein encoded by this gene functions as both a phosphodiesterase which cleaves phosphodiester bonds at the 5' end of oligonucleotides and a phospholipase which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Aug 2008]

Gene Symbol:   ENPP2
Gene Name:   ectonucleotide pyrophosphatase/phosphodiesterase 2
Aliases:   ATX, ATX-X, AUTOTAXIN, FLJ26803, LysoPLD, NPP2, PD-IALPHA, PDNP2
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000136960
Entrez:   5168
Chromosome Mapping:   8q24.1

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