PrimePCR™ Probe Assay: ESPL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ESPL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ESPL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032992
Assay Design:   Intron-spanning
Chromosome Location:   12:53673560-53675376question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000552671 ENST00000552462 ENST00000257934

Gene Information

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1 or separase which initiates the final separation of sister chromatids (Sun et al. 2009 [PubMed 19345191]).[supplied by OMIM Nov 2010]

Gene Symbol:   ESPL1
Gene Name:   extra spindle pole bodies homolog 1 (S. cerevisiae)
Aliases:   ESP1, FLJ46492, KIAA0165
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000135476
Entrez:   9700
Chromosome Mapping:   12q

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