PrimePCR™ Probe Assay: TIMP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TIMP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TIMP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033647
Assay Design:   Intron-spanning
Chromosome Location:   17:76853703-76869940question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000262768 ENST00000536189

Gene Information

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq Jul 2008]

Gene Symbol:   TIMP2
Gene Name:   TIMP metallopeptidase inhibitor 2
Aliases:   CSC-21K
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000035862
Entrez:   7077
Chromosome Mapping:   17q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.650000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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