PrimePCR™ Probe Assay: CHEK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CHEK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHEK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040478
Assay Design:   Intron-spanning
Chromosome Location:   11:125499286-125503126question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000427383 ENST00000428830 ENST00000527013 ENST00000526937 ENST00000534070 ENST00000524737 ENST00000532669 ENST00000278916 ENST00000438015 ENST00000544373

Gene Information

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR two cell cycle proteins involved in DNA damage responses that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   CHEK1
Gene Name:   CHK1 checkpoint homolog (S. pombe)
Aliases:   CHK1
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000149554
Entrez:   1111
Chromosome Mapping:   11q24.2

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