PrimePCR™ Probe Assay: Met, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053445
Assay Design:   exonic
Chromosome Location:   6:17546982-17548693question
Amplicon Length:   93
Splice Variants Targeted:   ENSMUST00000115443 ENSMUST00000115442 ENSMUST00000152802 ENSMUST00000148903 ENSMUST00000080469

Gene Information

Description Not Available

Gene Symbol:   Met
Gene Name:   Met proto-oncogene
Aliases:   AI838057, HGF, HGFR, Par4, c-Met
RefSeq:   NC_000072.6 NT_039340.7
Ensembl:   ENSMUSG00000009376
Entrez:   17295
Chromosome Mapping:   6 A2|6 4.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.860000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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