PrimePCR™ Probe Assay: YWHAZ, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: YWHAZ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: YWHAZ, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029093
Assay Design:   Intron-spanning
Chromosome Location:   8:101932945-101936435question
Amplicon Length:   175
Splice Variants Targeted:   ENST00000395957 ENST00000395956 ENST00000353245 ENST00000522542 ENST00000521309 ENST00000517797 ENST00000522819 ENST00000395953 ENST00000395948 ENST00000395951 ENST00000419477 ENST00000521607 ENST00000457309 ENST00000395958

Gene Information

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals and this protein is 99% identical to the mouse rat and sheep orthologs. The encoded protein interacts with IRS1 protein suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   YWHAZ
Gene Name:   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Aliases:   14-3-3-zeta, KCIP-1, MGC111427, MGC126532, MGC138156, YWHAD
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000164924
Entrez:   7534
Chromosome Mapping:   8q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.900000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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