PrimePCR™ Probe Assay: TINF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TINF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TINF2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025532
Assay Design:   Exonic
Chromosome Location:   14:24709681-24709802question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000538777 ENST00000267415 ENST00000540705 ENST00000399423

Gene Information

This gene encodes one of the proteins of the shelterin or telosome complex which protects telomeres by allowing the cell to distinguish between telomeres and regions of DNA damage. The protein encoded by this gene is a critical part of shelterin; it interacts with the three DNA-binding proteins of the shelterin complex and it is important for assembly of the complex. Mutations in this gene cause dyskeratosis congenita (DKC) an inherited bone marrow failure syndrome. [provided by RefSeq Mar 2010]

Gene Symbol:   TINF2
Gene Name:   TERF1 (TRF1)-interacting nuclear factor 2
Aliases:   TIN2
RefSeq:   NC_000014.8 NG_016650.1 NT_026437.12
Ensembl:   ENSG00000092330
Entrez:   26277
Chromosome Mapping:   14q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994400
y-intercept 36.280000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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