PrimePCR™ Probe Assay: MCL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MCL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MCL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039327
Assay Design:   exonic
Chromosome Location:   1:150549533-150549671question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000369026 ENST00000307940

Gene Information

This gene encodes an anti-apoptotic protein which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq Oct 2010]

Gene Symbol:   MCL1
Gene Name:   myeloid cell leukemia sequence 1 (BCL2-related)
Aliases:   BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, MGC104264, MGC1839, Mcl-1, TM, bcl2-L-3, mcl1/EAT
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143384
Entrez:   4170
Chromosome Mapping:   1q21

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