PrimePCR™ Probe Assay: HIST1H2AD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HIST1H2AD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HIST1H2AD, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039983
Assay Design:   exonic
Chromosome Location:   6:26199079-26199200question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000377831 ENST00000341023 ENST00000359193 ENST00000377459 ENST00000358739 ENST00000333151 ENST00000330180 ENST00000357320 ENST00000359611 ENST00000369161 ENST00000369159 ENST00000544848 ENST00000583968 ENST00000580456

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome an octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H2AD
Gene Name:   histone cluster 1, H2ad
Aliases:   H2A.3, H2A/g, H2AFG
RefSeq:   NC_000006.11 NG_001335.1 NT_007592.15
Ensembl:   ENSG00000196866
Entrez:   3013
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.920000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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