PrimePCR™ Probe Assay: HLA-E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HLA-E, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-E, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040072
Assay Design:   exonic
Chromosome Location:   6:30460560-30460703question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000427936 ENST00000383487 ENST00000383483 ENST00000458668 ENST00000400341 ENST00000437265 ENST00000421349 ENST00000553172 ENST00000444683 ENST00000383597 ENST00000415289 ENST00000376228 ENST00000383329 ENST00000539307 ENST00000453809 ENST00000414249 ENST00000419590 ENST00000438171 ENST00000552865 ENST00000412585 ENST00000436729 ENST00000435618 ENST00000546599 ENST00000376630 ENST00000415649 ENST00000425603 ENST00000419135 ENST00000433153 ENST00000429840 ENST00000457903 ENST00000425798 ENST

Gene Information

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide exons 2 and 3 encode the alpha1 and alpha2 domains which both bind the peptide exon 4 encodes the alpha3 domain exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-E
Gene Name:   major histocompatibility complex, class I, E
Aliases:   DKFZp686P19218, EA1.2, EA2.1, HLA-6.2, MHC, QA1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204592
Entrez:   3133
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 35.170000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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