PrimePCR™ Probe Assay: C4B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: C4B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: C4B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040144
Assay Design:   exonic
Chromosome Location:   6:31995079-31995469question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000375295 ENST00000546746 ENST00000449788 ENST00000546399 ENST00000411583 ENST00000548530 ENST00000383325 ENST00000549812 ENST00000428956 ENST00000498271 ENST00000435363 ENST00000425700 ENST00000435500 ENST00000552464 ENST00000375177 ENST00000548301 ENST00000406575 ENST00000420258 ENST00000421274 ENST00000550297 ENST00000357266 ENST00000536438 ENST00000539068 ENST00000540787 ENST00000542713

Gene Information

This gene encodes the basic form of complement factor 4 part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha beta and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist such that individuals may have 1 2 or 3 copies of this gene. In addition this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq Jul 2008]

Gene Symbol:   C4B
Gene Name:   complement component 4B (Chido blood group)
Aliases:   C4B1, C4B12, C4B2, C4B3, C4B5, C4F, CH, CO4, CPAMD3, FLJ60561, MGC164979
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NG_007941.2 NG_011639.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1
Ensembl:   ENSG00000224389
Entrez:   721
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.670000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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