PrimePCR™ Probe Assay: LTA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LTA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LTA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040152
Assay Design:   exonic
Chromosome Location:   6:31541502-31541629question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000426845 ENST00000412851 ENST00000371035 ENST00000436519 ENST00000454550 ENST00000454783 ENST00000418386 ENST00000456789 ENST00000448630 ENST00000432632 ENST00000441939 ENST00000383303 ENST00000383304 ENST00000400272 ENST00000383499

Gene Information

The encoded protein a member of the tumor necrosis factor family is a cytokine produced by lymphocytes. The protein is highly inducible secreted and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory immunostimulatory and antiviral responses is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.[provided by RefSeq Apr 2009]

Gene Symbol:   LTA
Gene Name:   lymphotoxin alpha (TNF superfamily, member 1)
Aliases:   LT, TNFB, TNFSF1
RefSeq:   NC_000006.11 NG_007462.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1 NG_012010.1
Ensembl:   ENSG00000226979
Entrez:   4049
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.930000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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