PrimePCR™ Probe Assay: SMAD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SMAD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SMAD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040455
Assay Design:   exonic
Chromosome Location:   4:146435635-146435790question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000514778 ENST00000507594 ENST00000514831 ENST00000302085 ENST00000512019 ENST00000503324 ENST00000507367 ENST00000394092 ENST00000515385

Gene Information

The protein encoded by this gene belongs to the SMAD a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs) which are involved in a range of biological activities including cell growth apoptosis morphogenesis development and immune responses. In response to BMP ligands this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4 which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases such as SMURF1 and SMURF2 and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq Jul 2008]

Gene Symbol:   SMAD1
Gene Name:   SMAD family member 1
Aliases:   BSP1, JV4-1, JV41, MADH1, MADR1
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000170365
Entrez:   4086
Chromosome Mapping:   4q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.460000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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