PrimePCR™ Probe Assay: KRT8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KRT8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KRT8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041467
Assay Design:   exonic
Chromosome Location:   12:53294372-53294465question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000331817 ENST00000252245 ENST00000252252 ENST00000252250 ENST00000409686 ENST00000330722 ENST00000417996 ENST00000309505 ENST00000551956 ENST00000293774 ENST00000458244 ENST00000552551 ENST00000293308 ENST00000546897 ENST00000552150 ENST00000546826 ENST00000547176 ENST00000548998 ENST00000546900

Gene Information

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jan 2012]

Gene Symbol:   KRT8
Gene Name:   keratin 8
Aliases:   CARD2, CK8, CYK8, K2C8, K8, KO
RefSeq:   NC_000012.11 NG_008402.1 NT_029419.12
Ensembl:   ENSG00000170421
Entrez:   3856
Chromosome Mapping:   12q13

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