PrimePCR™ Probe Assay: SCP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SCP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SCP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041611
Assay Design:   exonic
Chromosome Location:   1:53516434-53516553question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000371514 ENST00000528311 ENST00000371509 ENST00000407246 ENST00000430330 ENST00000484100 ENST00000435345 ENST00000488965 ENST00000408941

Gene Information

This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2) as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein and the transcript initiated from the distal promoter encodes the shorter SCP2 protein with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism and may play a role in Zellweger syndrome in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants some encoding different isoforms.[provided by RefSeq Aug 2010]

Gene Symbol:   SCP2
Gene Name:   sterol carrier protein 2
Aliases:   DKFZp686C12188, DKFZp686D11188, NLTP, NSL-TP, SCP-2, SCP-CHI, SCP-X, SCPX
RefSeq:   NC_000001.10 NT_032977.9 NG_012211.1
Ensembl:   ENSG00000116171
Entrez:   6342
Chromosome Mapping:   1p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 37.200000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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