PrimePCR™ Probe Assay: TCF7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TCF7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TCF7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041621
Assay Design:   exonic
Chromosome Location:   5:133451600-133451689question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000432532 ENST00000378564 ENST00000321584 ENST00000321603 ENST00000395023 ENST00000518915 ENST00000520958 ENST00000378560 ENST00000522375 ENST00000521639 ENST00000517851 ENST00000518887 ENST00000395029 ENST00000342854

Gene Information

The protein encoded by this gene is a transcriptional activator that plays an important role in lymphocyte differentiation. This gene is expressed predominantly in T-cells. The encoded protein can bind an enhancer element and activate the CD3E gene and it also may repress the CTNNB1 and TCF7L2 genes through a feedback mechanism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   TCF7
Gene Name:   transcription factor 7 (T-cell specific, HMG-box)
Aliases:   FLJ36364, MGC47735, TCF-1
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000081059
Entrez:   6932
Chromosome Mapping:   5q31.1

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