PrimePCR™ Probe Assay: ARAF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ARAF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARAF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049671
Assay Design:   exonic
Chromosome Location:   X:47424407-47424666question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000377045 ENST00000377039 ENST00000290277

Gene Information

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jan 2012]

Gene Symbol:   ARAF
Gene Name:   v-raf murine sarcoma 3611 viral oncogene homolog
Aliases:   A-RAF, ARAF1, PKS2, RAFA1
RefSeq:   NC_000023.10 NG_008437.1 NG_016339.1 NT_079573.4
Ensembl:   ENSG00000078061
Entrez:   369
Chromosome Mapping:   Xp11.4-p11.2

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