PrimePCR™ Probe Assay: PTHLH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTHLH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTHLH, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049830
Assay Design:   exonic
Chromosome Location:   12:28116578-28116669question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000539239 ENST00000545234 ENST00000538310 ENST00000535992 ENST00000395868 ENST00000542963 ENST00000534890 ENST00000395872 ENST00000354417 ENST00000201015

Gene Information

The protein encoded by this gene is a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone PTHR1 is responsible for most cases of humoral hypercalcemia of malignancy. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites in-frame and downstream of the initiator AUG codon to give rise to nuclear forms of this hormone. [provided by RefSeq Jul 2008]

Gene Symbol:   PTHLH
Gene Name:   parathyroid hormone-like hormone
Aliases:   BDE2, HHM, MGC14611, PLP, PTHR, PTHRP
RefSeq:   NC_000012.11 NT_009714.17 NG_023197.1
Ensembl:   ENSG00000087494
Entrez:   5744
Chromosome Mapping:   12p12.1-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.080000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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