PrimePCR™ Probe Assay: MCM3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MCM3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MCM3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050683
Assay Design:   exonic
Chromosome Location:   6:52129414-52129535question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000419835 ENST00000229854 ENST00000421471 ENST00000596288

Gene Information

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and thus is acetlyated by MCM3AP a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq Jul 2008]

Gene Symbol:   MCM3
Gene Name:   minichromosome maintenance complex component 3
Aliases:   HCC5, MGC1157, P1-MCM3, P1.h, RLFB
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112118
Entrez:   4172
Chromosome Mapping:   6p12

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